Journal: BMC Gastroenterology

Article Title: Triptolide attenuates irritable bowel syndrome via inhibiting ODC1

doi: 10.1186/s12876-023-02847-8

Figure Lengend Snippet: The effects of triptolide administration on behavioral symptoms in rats with CAS-inudced IBS. The the marbleburying tests (A) and immobility time in the forced swimming (B) were performed to investigate anxiety- and depressant- like behaviors. The rats were either subjected to chronic acute combined stress for 35 days or left undisturbed (control group). Results are expressed as mean ± SD (n = 6). * P < 0.05 and ** P < 0.01 vs. Control group

Article Snippet: Triptolide was purchased from MedChemExpress, which was dissolved in DMSO and diluted with olive oil.

Techniques: Control

Journal: BMC Gastroenterology

Article Title: Triptolide attenuates irritable bowel syndrome via inhibiting ODC1

doi: 10.1186/s12876-023-02847-8

Figure Lengend Snippet: The effects of triptolide administration on visceral hypersensitivity of bowel in rats with CAS-inudced IBS. The fecal weight (A), n = 4 and AWR score in the CRD testing (B), n = 6 was carried out to evaluate visceral hypersensitivity of bowel. The rats were either subjected to chronic acute combined stress for 35 days or left undisturbed (Control group). Results are expressed as mean ± SD. ** P < 0.01 vs. Control group, # P < 0.05 and ## P < 0.01 vs. IBS group. CRD, colorectal distension; AWR, abdominal withdrawal reflex

Article Snippet: Triptolide was purchased from MedChemExpress, which was dissolved in DMSO and diluted with olive oil.

Techniques: Control

Journal: BMC Gastroenterology

Article Title: Triptolide attenuates irritable bowel syndrome via inhibiting ODC1

doi: 10.1186/s12876-023-02847-8

Figure Lengend Snippet: The intestinal structural changes after treatment with triptolide in rats with CAS-inudced IBS. HE was used to stain the ileum and colon of rats in each group (40X). HE, hematoxylin and eosin

Article Snippet: Triptolide was purchased from MedChemExpress, which was dissolved in DMSO and diluted with olive oil.

Techniques: Staining

Journal: BMC Gastroenterology

Article Title: Triptolide attenuates irritable bowel syndrome via inhibiting ODC1

doi: 10.1186/s12876-023-02847-8

Figure Lengend Snippet: The effects of triptolide administration on inflammatory cytokines expression in rats with CAS-inudced IBS. ELISA was conducted to measure the expression of IL-1, IL-6, and TNF-α in the ileum (A) and colon (B) in each group. Results are expressed as mean ± SD (n = 6). ** P < 0.01 vs. Control group, ## P < 0.01 vs. IBS group

Article Snippet: Triptolide was purchased from MedChemExpress, which was dissolved in DMSO and diluted with olive oil.

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Control

Journal: BMC Gastroenterology

Article Title: Triptolide attenuates irritable bowel syndrome via inhibiting ODC1

doi: 10.1186/s12876-023-02847-8

Figure Lengend Snippet: The effects of triptolide administration on ODC expression in rats with CAS-inudced IBS. Western blot analysis was carried out to detect the protein expression of ODC in the ileum (A) and colon (B) normalized to GAPDH. The samples derive from the same experiment and that blots were processed in parallel. Results are expressed as mean ± SD (n = 3). * P < 0.05, ** P < 0.01 vs. Control group, and # P < 0.05, ## P < 0.01 vs. IBS group

Article Snippet: Triptolide was purchased from MedChemExpress, which was dissolved in DMSO and diluted with olive oil.

Techniques: Expressing, Western Blot, Control